- Catalog number
- EK2152
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- HLA class II histocompatibility antigen gamma chain also known as HLA-DR antigens-associated invariant chain or CD74 (Cluster of Differentiation 74), is a protein that in humans is encoded by the CD74 gene. The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta) . Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 296 311 279 291 294 16.18 5.5% Sample 2 1326 1344 1495 1451 1404 78.62 5.6% Sample 3 4896 4886 4612 4545 4735 326.7 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 296 311 279 291 294 16.18 5.5% Sample 1 296 311 279 291 294 16.18 5.5% Sample 2 1326 1344 1495 1451 1404 78.62 5.6% Sample 2 1326 1344 1495 1451 1404 78.62 5.6% Sample 3 4896 4886 4612 4545 4735 326.7 6.9% Sample 3 4896 4886 4612 4545 4735 326.7 6.9%
- Gene Name
- CD74
- Gene ID
- 972
- UniProt
- P04233
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: Q73-M232
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 296 1326 4896 292 1423 4866 Standard deviation 12.73 80.89 318.24 20.73 83.96 311.42 CV (%) 4.3% 6.1% 6.5% 7.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 296 1326 4896 292 1423 4866 Standard deviation 12.73 80.89 318.24 20.73 83.96 311.42 CV (%) 4.3% 6.1% 6.5% 7.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 296 1326 4896 292 1423 4866 Mean (pg/ml) 296 1326 4896 292 1423 4866 Standard deviation 12.73 80.89 318.24 20.73 83.96 311.42 Standard deviation 12.73 80.89 318.24 20.73 83.96 311.42 CV (%) 4.3% 6.1% 6.5% 7.1% 5.9% 6.4% CV (%) 4.3% 6.1% 6.5% 7.1% 5.9% 6.4%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CD74
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.065 0.123 0.173 0.261 0.475 0.845 1.441 2.343 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.065 0.123 0.173 0.261 0.475 0.845 1.441 2.343 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.065 0.123 0.173 0.261 0.475 0.845 1.441 2.343 O.D. 0.065 0.123 0.173 0.261 0.475 0.845 1.441 2.343
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
