- Catalog number
- EK2153
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD79b molecule, immunoglobulin-associated beta, also known as CD79B (Cluster of Differentiation 79B), is a human gene. The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig) . Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 39 41 40 41 2.3 5.6% Sample 2 235 253 256 227 243 10.2 4.2% Sample 3 453 528 500 506 497 25.83 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 39 41 40 41 2.3 5.6% Sample 1 44 39 41 40 41 2.3 5.6% Sample 2 235 253 256 227 243 10.2 4.2% Sample 2 235 253 256 227 243 10.2 4.2% Sample 3 453 528 500 506 497 25.83 5.2% Sample 3 453 528 500 506 497 25.83 5.2%
- Gene Name
- CD79B
- Gene ID
- 974
- UniProt
- P40259
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: A29-D159
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 235 453 47 240 445 Standard deviation 2.9 18.1 25.37 2.77 14.88 30.26 CV (%) 6.6% 7.7% 5.6% 5.9% 6.2% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 235 453 47 240 445 Standard deviation 2.9 18.1 25.37 2.77 14.88 30.26 CV (%) 6.6% 7.7% 5.6% 5.9% 6.2% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 44 235 453 47 240 445 Mean (pg/ml) 44 235 453 47 240 445 Standard deviation 2.9 18.1 25.37 2.77 14.88 30.26 Standard deviation 2.9 18.1 25.37 2.77 14.88 30.26 CV (%) 6.6% 7.7% 5.6% 5.9% 6.2% 6.8% CV (%) 6.6% 7.7% 5.6% 5.9% 6.2% 6.8%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CD79B
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.061 0.128 0.236 0.412 0.835 1.39 2.107 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.061 0.128 0.236 0.412 0.835 1.39 2.107 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.061 0.128 0.236 0.412 0.835 1.39 2.107 O.D. 0.02 0.061 0.128 0.236 0.412 0.835 1.39 2.107
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
