Human CD302 ELISA Kit PicoKine®

About this product

Human CD302 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD302 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2156

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

The CD302 antigen also known as C-type lectin domain family 13 member A is a protein that in humans is encoded by the CD302 gene. CD302 is a C-type lectin receptor involved in cell adhesion and migration, as well as endocytosis and phagocytosis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 136 142 133 129 135 6.62 4.9% Sample 2 252 258 261 274 261 9.41 3.6% Sample 3 548 569 582 603 576 18.42 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 136 142 133 129 135 6.62 4.9% Sample 1 136 142 133 129 135 6.62 4.9% Sample 2 252 258 261 274 261 9.41 3.6% Sample 2 252 258 261 274 261 9.41 3.6% Sample 3 548 569 582 603 576 18.42 3.2% Sample 3 548 569 582 603 576 18.42 3.2%

Gene Name

CD302

Gene ID

9936

UniProt

Q8IX05

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: HEK293; Immunogen sequence: D23-N167

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 136 252 548 147 274 559 Standard deviation 5.98 13.1 18.08 7.2 16.71 30.19 CV (%) 4.4% 5.2% 3.3% 4.9% 6.1% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 136 252 548 147 274 559 Standard deviation 5.98 13.1 18.08 7.2 16.71 30.19 CV (%) 4.4% 5.2% 3.3% 4.9% 6.1% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 136 252 548 147 274 559 Mean (pg/ml) 136 252 548 147 274 559 Standard deviation 5.98 13.1 18.08 7.2 16.71 30.19 Standard deviation 5.98 13.1 18.08 7.2 16.71 30.19 CV (%) 4.4% 5.2% 3.3% 4.9% 6.1% 5.4% CV (%) 4.4% 5.2% 3.3% 4.9% 6.1% 5.4%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD302

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.062 0.103 0.171 0.365 0.665 1.155 1.854 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.062 0.103 0.171 0.365 0.665 1.155 1.854 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.062 0.103 0.171 0.365 0.665 1.155 1.854 O.D. 0.021 0.062 0.103 0.171 0.365 0.665 1.155 1.854

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.