Human CDO ELISA Kit PicoKine®

About this product

Human CDO ELISA Kit PicoKine® (96 Tests) . Quantitate Human CDON in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2157

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cell adhesion molecule-related/down-regulated by oncogenes is a protein that in humans is encoded by the CDON gene. This gene encodes a cell surface receptor that is a member of the immunoglobulin superfamily. The encoded protein contains three fibronectin type III domains and five immunoglobulin-like C2-type domains. This protein is a member of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells and positively regulates myogenesis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 202 236 212 233 221 13.47 6.1% Sample 2 1756 1788 1863 1923 1833 69.64 3.8% Sample 3 4943 4465 4863 4812 4771 248.08 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 202 236 212 233 221 13.47 6.1% Sample 1 202 236 212 233 221 13.47 6.1% Sample 2 1756 1788 1863 1923 1833 69.64 3.8% Sample 2 1756 1788 1863 1923 1833 69.64 3.8% Sample 3 4943 4465 4863 4812 4771 248.08 5.2% Sample 3 4943 4465 4863 4812 4771 248.08 5.2%

Gene Name

CDON

Gene ID

50937

UniProt

Q4KMG0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D26-P943

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 202 1756 4943 211 1963 4861 Standard deviation 12.52 133.46 370.73 14.56 113.85 286.8 CV (%) 6.2% 7.6% 7.5% 6.9% 5.8% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 202 1756 4943 211 1963 4861 Standard deviation 12.52 133.46 370.73 14.56 113.85 286.8 CV (%) 6.2% 7.6% 7.5% 6.9% 5.8% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 202 1756 4943 211 1963 4861 Mean (pg/ml) 202 1756 4943 211 1963 4861 Standard deviation 12.52 133.46 370.73 14.56 113.85 286.8 Standard deviation 12.52 133.46 370.73 14.56 113.85 286.8 CV (%) 6.2% 7.6% 7.5% 6.9% 5.8% 5.9% CV (%) 6.2% 7.6% 7.5% 6.9% 5.8% 5.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CDON

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.144 0.255 0.45 0.831 1.445 2.087 2.562 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.144 0.255 0.45 0.831 1.445 2.087 2.562 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.144 0.255 0.45 0.831 1.445 2.087 2.562 O.D. 0.016 0.144 0.255 0.45 0.831 1.445 2.087 2.562

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.