Human Langerin/CD207 ELISA Kit PicoKine®

About this product

Human Langerin/CD207 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD207 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2162

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Langerin (CD207) is a type II transmembrane protein which is encoded by the CD207 gene in humans. The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in this gene result in Birbeck granules deficiency or loss of sugar binding activity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 195 198 205 193 10.96 5.6% Sample 2 1366 1296 1142 1223 1257 74.15 5.9% Sample 3 5456 6236 5869 57415 5826 262.15 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 195 198 205 193 10.96 5.6% Sample 1 185 195 198 205 193 10.96 5.6% Sample 2 1366 1296 1142 1223 1257 74.15 5.9% Sample 2 1366 1296 1142 1223 1257 74.15 5.9% Sample 3 5456 6236 5869 57415 5826 262.15 4.5% Sample 3 5456 6236 5869 57415 5826 262.15 4.5%

Gene Name

CD207

Gene ID

50489

UniProt

Q9UJ71

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: P65-P328

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 1366 5456 183 1469 5812 Standard deviation 8.88 88.79 349.18 12.63 113.11 412.65 CV (%) 4.8% 6.5% 6.4% 6.9% 7.7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 1366 5456 183 1469 5812 Standard deviation 8.88 88.79 349.18 12.63 113.11 412.65 CV (%) 4.8% 6.5% 6.4% 6.9% 7.7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 185 1366 5456 183 1469 5812 Mean (pg/ml) 185 1366 5456 183 1469 5812 Standard deviation 8.88 88.79 349.18 12.63 113.11 412.65 Standard deviation 8.88 88.79 349.18 12.63 113.11 412.65 CV (%) 4.8% 6.5% 6.4% 6.9% 7.7% 7.1% CV (%) 4.8% 6.5% 6.4% 6.9% 7.7% 7.1%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD207

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.094 0.145 0.252 0.43 0.759 1.388 2.044 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.094 0.145 0.252 0.43 0.759 1.388 2.044 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.094 0.145 0.252 0.43 0.759 1.388 2.044 O.D. 0.025 0.094 0.145 0.252 0.43 0.759 1.388 2.044

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.