Human Chondroadherin ELISA Kit PicoKine®

About this product

Human Chondroadherin ELISA Kit PicoKine® (96 Tests) . Quantitate Human CHAD in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2167

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chondroadherin is a cartilage matrix protein thought to mediate adhesion of isolated chondrocytes. The protein contains 11 leucine-rich repeats flanked by cysteine-rich regions. The chondroadherin messenger RNA is present in chondrocytes at all ages.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 541 562 601 612 579 25.48 4.4% Sample 2 2865 2954 2811 2826 2864 83.06 2.9% Sample 3 7643 7263 8106 7576 7647 290.59 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 541 562 601 612 579 25.48 4.4% Sample 1 541 562 601 612 579 25.48 4.4% Sample 2 2865 2954 2811 2826 2864 83.06 2.9% Sample 2 2865 2954 2811 2826 2864 83.06 2.9% Sample 3 7643 7263 8106 7576 7647 290.59 3.8% Sample 3 7643 7263 8106 7576 7647 290.59 3.8%

Gene Name

CHAD

Gene ID

1101

UniProt

O15335

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C23-H359

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 541 2865 7643 522 2972 7652 Standard deviation 27.59 100.28 298.08 21.4 127.8 359.64 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 541 2865 7643 522 2972 7652 Standard deviation 27.59 100.28 298.08 21.4 127.8 359.64 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 541 2865 7643 522 2972 7652 Mean (pg/ml) 541 2865 7643 522 2972 7652 Standard deviation 27.59 100.28 298.08 21.4 127.8 359.64 Standard deviation 27.59 100.28 298.08 21.4 127.8 359.64 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7%

Sensitivity

<20 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CHAD

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.0 0.086 0.157 0.277 0.528 0.961 1.64 2.286 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.0 0.086 0.157 0.277 0.528 0.961 1.64 2.286 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.0 0.086 0.157 0.277 0.528 0.961 1.64 2.286 O.D. 0.0 0.086 0.157 0.277 0.528 0.961 1.64 2.286

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.