Human CHRDL2 ELISA Kit PicoKine®

About this product

Human CHRDL2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CHRDL2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2168

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Pro-collagen

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 158 299 270 258 19.20 7.5% Sample 2 685 772 1004 1189 913 46.45 5.1% Sample 3 2742 3304 4400 4538 3746 119.87 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 158 299 270 258 19.20 7.5% Sample 1 304 158 299 270 258 19.20 7.5% Sample 2 685 772 1004 1189 913 46.45 5.1% Sample 2 685 772 1004 1189 913 46.45 5.1% Sample 3 2742 3304 4400 4538 3746 119.87 3.2% Sample 3 2742 3304 4400 4538 3746 119.87 3.2%

Gene Name

CHRDL2

Gene ID

25884

UniProt

Q6WN34

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-T429

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156pg/mL - 10000pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 685 2742 261 625 3040 Standard deviation 6.48 14.93 137.92 9.27 29.75 98.50 CV (%) 2.1% 2.2% 5.0% 3.6% 4.8% 3.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 685 2742 261 625 3040 Standard deviation 6.48 14.93 137.92 9.27 29.75 98.50 CV (%) 2.1% 2.2% 5.0% 3.6% 4.8% 3.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 304 685 2742 261 625 3040 Mean (pg/ml) 304 685 2742 261 625 3040 Standard deviation 6.48 14.93 137.92 9.27 29.75 98.50 Standard deviation 6.48 14.93 137.92 9.27 29.75 98.50 CV (%) 2.1% 2.2% 5.0% 3.6% 4.8% 3.2% CV (%) 2.1% 2.2% 5.0% 3.6% 4.8% 3.2%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CHRDL2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 250 5000 10000 O.D. 0.003 0.087 0.177 0.313 0.558 0.926 1.491 2.357 Concentration (pg/ml) 0 156 312 625 1250 250 5000 10000 O.D. 0.003 0.087 0.177 0.313 0.558 0.926 1.491 2.357 Concentration (pg/ml) 0 156 312 625 1250 250 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 250 5000 10000 O.D. 0.003 0.087 0.177 0.313 0.558 0.926 1.491 2.357 O.D. 0.003 0.087 0.177 0.313 0.558 0.926 1.491 2.357

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.