Mouse IL-18 ELISA Kit PicoKine®

About this product

Mouse IL-18 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il18 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0433

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin (IL) -18

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 54 57 60 56 1.44 2.6% Sample 2 286 302 292 297 294 9.12 3.1% Sample 3 905 880 869 892 887 24.42 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 54 57 60 56 1.44 2.6% Sample 1 51 54 57 60 56 1.44 2.6% Sample 2 286 302 292 297 294 9.12 3.1% Sample 2 286 302 292 297 294 9.12 3.1% Sample 3 905 880 869 892 887 24.42 2.8% Sample 3 905 880 869 892 887 24.42 2.8%

Gene Name

Il18

Gene ID

16173

UniProt

P70380

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N36-S192

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 286 905 59 310 933 Standard deviation 2.14 11.15 41.63 3.07 17.05 45.72 CV (%) 4.2% 3.9% 4.6% 5.2% 5.5% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 286 905 59 310 933 Standard deviation 2.14 11.15 41.63 3.07 17.05 45.72 CV (%) 4.2% 3.9% 4.6% 5.2% 5.5% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 51 286 905 59 310 933 Mean (pg/ml) 51 286 905 59 310 933 Standard deviation 2.14 11.15 41.63 3.07 17.05 45.72 Standard deviation 2.14 11.15 41.63 3.07 17.05 45.72 CV (%) 4.2% 3.9% 4.6% 5.2% 5.5% 4.9% CV (%) 4.2% 3.9% 4.6% 5.2% 5.5% 4.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Mouse Il18

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.1 0.166 0.267 0.467 0.812 1.348 2.138 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.1 0.166 0.267 0.467 0.812 1.348 2.138 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.1 0.166 0.267 0.467 0.812 1.348 2.138 O.D. 0.023 0.1 0.166 0.267 0.467 0.812 1.348 2.138

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.