Human CRTAM ELISA Kit PicoKine®

About this product

Human CRTAM ELISA Kit PicoKine® (96 Tests) . Quantitate Human CRTAM in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2172

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

The CRTAM gene is upregulated in CD4-positive and CD8-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 38 41 45 44 1.95 4.4% Sample 2 284 285 271 266 277 8.85 3.2% Sample 3 914 898 906 923 910 44.60 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 38 41 45 44 1.95 4.4% Sample 1 58 38 41 45 44 1.95 4.4% Sample 2 284 285 271 266 277 8.85 3.2% Sample 2 284 285 271 266 277 8.85 3.2% Sample 3 914 898 906 923 910 44.60 4.9% Sample 3 914 898 906 923 910 44.60 4.9%

Gene Name

CRTAM

Gene ID

56253

UniProt

O95727

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S18-S286

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 284 914 51 322 942 Standard deviation 2.28 10.79 44.79 2.65 18.03 48.04 CV (%) 4.3% 3.8% 4.9% 5.2% 5.6% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 284 914 51 322 942 Standard deviation 2.28 10.79 44.79 2.65 18.03 48.04 CV (%) 4.3% 3.8% 4.9% 5.2% 5.6% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 53 284 914 51 322 942 Mean (pg/ml) 53 284 914 51 322 942 Standard deviation 2.28 10.79 44.79 2.65 18.03 48.04 Standard deviation 2.28 10.79 44.79 2.65 18.03 48.04 CV (%) 4.3% 3.8% 4.9% 5.2% 5.6% 5.1% CV (%) 4.3% 3.8% 4.9% 5.2% 5.6% 5.1%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Human CRTAM

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.147 0.228 0.38 0.62 1.066 1.778 2.793 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.147 0.228 0.38 0.62 1.066 1.778 2.793 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.147 0.228 0.38 0.62 1.066 1.778 2.793 O.D. 0.028 0.147 0.228 0.38 0.62 1.066 1.778 2.793

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.