Human CSPG5 ELISA Kit PicoKine®

About this product

Human CSPG5 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CSPG5 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2173

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chondroitin sulfate proteoglycan 5 is a protein that in humans is encoded by the CSPG5 gene. The protein encoded by this gene is a proteoglycan that may function as a neural growth and differentiation factor. Several transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 33 40 42 38 1.86 4.9% Sample 2 261 263 259 272 264 16.35 6.2% Sample 3 412 433 436 425 427 27.72 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 33 40 42 38 1.86 4.9% Sample 1 37 33 40 42 38 1.86 4.9% Sample 2 261 263 259 272 264 16.35 6.2% Sample 2 261 263 259 272 264 16.35 6.2% Sample 3 412 433 436 425 427 27.72 6.5% Sample 3 412 433 436 425 427 27.72 6.5%

Gene Name

CSPG5

Gene ID

10675

UniProt

O95196

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: V31-Q420

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 261 412 39 269 377 Standard deviation 2.33 15.40 19.36 2.61 19.64 21.87 CV (%) 6.3% 5.9% 4.7% 6.7% 7.3% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 261 412 39 269 377 Standard deviation 2.33 15.40 19.36 2.61 19.64 21.87 CV (%) 6.3% 5.9% 4.7% 6.7% 7.3% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 261 412 39 269 377 Mean (pg/ml) 37 261 412 39 269 377 Standard deviation 2.33 15.40 19.36 2.61 19.64 21.87 Standard deviation 2.33 15.40 19.36 2.61 19.64 21.87 CV (%) 6.3% 5.9% 4.7% 6.7% 7.3% 5.8% CV (%) 6.3% 5.9% 4.7% 6.7% 7.3% 5.8%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Human CSPG5

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.059 0.131 0.176 0.277 0.461 0.836 1.439 2.2 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.059 0.131 0.176 0.277 0.461 0.836 1.439 2.2 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.059 0.131 0.176 0.277 0.461 0.836 1.439 2.2 O.D. 0.059 0.131 0.176 0.277 0.461 0.836 1.439 2.2

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.