Human CLEC5A ELISA Kit PicoKine®

About this product

Human CLEC5A ELISA Kit PicoKine® (96 Tests) . Quantitate Human CLEC5A in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2175

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

C-type lectin domain family 5 member A (CLEC5A), also known as C-type lectin superfamily member 5 (CLECSF5) and myeloid DAP12-associating lectin 1 (MDL-1) is a C-type lectin that in humans is encoded by the CLEC5A gene. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein interacts with dnax-activation protein 12 and may play a role in cell activation. Alternative splice variants have been described but their full-length sequence has not been determined.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 51 53 52 53 1.37 2.6% Sample 2 278 282 269 277 277 9.12 3.3% Sample 3 903 884 896 864 887 33.7 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 51 53 52 53 1.37 2.6% Sample 1 55 51 53 52 53 1.37 2.6% Sample 2 278 282 269 277 277 9.12 3.3% Sample 2 278 282 269 277 277 9.12 3.3% Sample 3 903 884 896 864 887 33.7 3.8% Sample 3 903 884 896 864 887 33.7 3.8%

Gene Name

CLEC5A

Gene ID

23601

UniProt

Q9NY25

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y26-K188

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 278 903 58 312 936 Standard deviation 1.98 9.17 37.02 2.96 15.29 39.31 CV (%) 3.6% 3.3% 4.1% 5.1% 4.9% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 278 903 58 312 936 Standard deviation 1.98 9.17 37.02 2.96 15.29 39.31 CV (%) 3.6% 3.3% 4.1% 5.1% 4.9% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 278 903 58 312 936 Mean (pg/ml) 55 278 903 58 312 936 Standard deviation 1.98 9.17 37.02 2.96 15.29 39.31 Standard deviation 1.98 9.17 37.02 2.96 15.29 39.31 CV (%) 3.6% 3.3% 4.1% 5.1% 4.9% 4.2% CV (%) 3.6% 3.3% 4.1% 5.1% 4.9% 4.2%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Human CLEC5A

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.158 0.244 0.395 0.629 1.029 1.635 2.464 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.158 0.244 0.395 0.629 1.029 1.635 2.464 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.158 0.244 0.395 0.629 1.029 1.635 2.464 O.D. 0.027 0.158 0.244 0.395 0.629 1.029 1.635 2.464

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.