- Catalog number
- EK2180
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine [L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 546 565 542 551 551 23.69 4.3% Sample 2 1734 1726 1754 1771 1746 80.33 4.6% Sample 3 7234 7302 7165 7139 7210 410.97 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 546 565 542 551 551 23.69 4.3% Sample 1 546 565 542 551 551 23.69 4.3% Sample 2 1734 1726 1754 1771 1746 80.33 4.6% Sample 2 1734 1726 1754 1771 1746 80.33 4.6% Sample 3 7234 7302 7165 7139 7210 410.97 5.7% Sample 3 7234 7302 7165 7139 7210 410.97 5.7%
- Gene Name
- CPB1
- Gene ID
- 1360
- UniProt
- P15086
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: H16-Y417
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 546 1734 7264 549 1753 7636 Standard deviation 34.4 104.04 450.37 34.59 103.43 465.8 CV (%) 6.3% 6% 6.2% 6.3% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 546 1734 7264 549 1753 7636 Standard deviation 34.4 104.04 450.37 34.59 103.43 465.8 CV (%) 6.3% 6% 6.2% 6.3% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 546 1734 7264 549 1753 7636 Mean (pg/ml) 546 1734 7264 549 1753 7636 Standard deviation 34.4 104.04 450.37 34.59 103.43 465.8 Standard deviation 34.4 104.04 450.37 34.59 103.43 465.8 CV (%) 6.3% 6% 6.2% 6.3% 5.9% 6.1% CV (%) 6.3% 6% 6.2% 6.3% 5.9% 6.1%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant Human CPB1
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.015 0.094 0.168 0.291 0.441 0.762 1.267 1.857 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.015 0.094 0.168 0.291 0.441 0.762 1.267 1.857 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.015 0.094 0.168 0.291 0.441 0.762 1.267 1.857 O.D. 0.015 0.094 0.168 0.291 0.441 0.762 1.267 1.857
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
