- Catalog number
- EK2181
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Carboxypeptidase M (EC 3.4.17.12, CPM) is an enzyme.The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 112 108 103 107 2.78 2.6% Sample 2 468 453 450 466 459 16.07 3.5% Sample 3 1752 1821 1796 1804 1793 55.59 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 112 108 103 107 2.78 2.6% Sample 1 105 112 108 103 107 2.78 2.6% Sample 2 468 453 450 466 459 16.07 3.5% Sample 2 468 453 450 466 459 16.07 3.5% Sample 3 1752 1821 1796 1804 1793 55.59 3.1% Sample 3 1752 1821 1796 1804 1793 55.59 3.1%
- Gene Name
- CPM
- Gene ID
- 1368
- UniProt
- P14384
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: L18-S423
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 468 1752 123 470 1825 Standard deviation 5.36 16.38 68.33 5.04 20.21 85.78 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 468 1752 123 470 1825 Standard deviation 5.36 16.38 68.33 5.04 20.21 85.78 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 105 468 1752 123 470 1825 Mean (pg/ml) 105 468 1752 123 470 1825 Standard deviation 5.36 16.38 68.33 5.04 20.21 85.78 Standard deviation 5.36 16.38 68.33 5.04 20.21 85.78 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant Human CPM
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.074 0.105 0.209 0.377 0.702 1.248 1.927 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.074 0.105 0.209 0.377 0.702 1.248 1.927 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.074 0.105 0.209 0.377 0.702 1.248 1.927 O.D. 0.004 0.074 0.105 0.209 0.377 0.702 1.248 1.927
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
