- Catalog number
- EK2183
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Discoidin, CUB and LCCL domain-containing protein 2 is a protein that in humans is encoded by the DCBLD2 gene. Involved in negative regulation of cell growth and wound healing. Located in cell surface. Is integral component of plasma membrane.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 109 98 123 111 5 4.5% Sample 2 435 441 452 462 448 22.82 5.1% Sample 3 1216 1251 1263 1196 1232 68.96 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 109 98 123 111 5 4.5% Sample 1 114 109 98 123 111 5 4.5% Sample 2 435 441 452 462 448 22.82 5.1% Sample 2 435 441 452 462 448 22.82 5.1% Sample 3 1216 1251 1263 1196 1232 68.96 5.6% Sample 3 1216 1251 1263 1196 1232 68.96 5.6%
- Gene Name
- DCBLD2
- Gene ID
- 131566
- UniProt
- Q96PD2
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q67-A528
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 435 1216 120 441 1268 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 435 1216 120 441 1268 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 114 435 1216 120 441 1268 Mean (pg/ml) 114 435 1216 120 441 1268 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8% CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant Human DCBLD2
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
