Human DDAH1 ELISA Kit PicoKine®

About this product

Human DDAH1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human DDAH1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2185

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 48 42 40 44 2.02 4.6% Sample 2 239 236 255 241 243 12.38 5.1% Sample 3 452 512 500 495 490 25.96 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 48 42 40 44 2.02 4.6% Sample 1 46 48 42 40 44 2.02 4.6% Sample 2 239 236 255 241 243 12.38 5.1% Sample 2 239 236 255 241 243 12.38 5.1% Sample 3 452 512 500 495 490 25.96 5.3% Sample 3 452 512 500 495 490 25.96 5.3%

Gene Name

DDAH1

Gene ID

23576

UniProt

O94760

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-S285

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 239 452 49 241 455 Standard deviation 2.58 12.19 19.44 1.91 14.94 29.58 CV (%) 5.6% 5.1% 4.3% 3.9% 6.2% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 239 452 49 241 455 Standard deviation 2.58 12.19 19.44 1.91 14.94 29.58 CV (%) 5.6% 5.1% 4.3% 3.9% 6.2% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 46 239 452 49 241 455 Mean (pg/ml) 46 239 452 49 241 455 Standard deviation 2.58 12.19 19.44 1.91 14.94 29.58 Standard deviation 2.58 12.19 19.44 1.91 14.94 29.58 CV (%) 5.6% 5.1% 4.3% 3.9% 6.2% 6.5% CV (%) 5.6% 5.1% 4.3% 3.9% 6.2% 6.5%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Human DDAH1

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.109 0.181 0.33 0.593 1.06 1.864 2.415 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.109 0.181 0.33 0.593 1.06 1.864 2.415 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.109 0.181 0.33 0.593 1.06 1.864 2.415 O.D. 0.02 0.109 0.181 0.33 0.593 1.06 1.864 2.415

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.