Human DPP7 ELISA Kit PicoKine®

About this product

Human DPP7 ELISA Kit PicoKine® (96 Tests) . Quantitate Human DPP7 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2186

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dipeptidyl-peptidase 2 is an enzyme that in humans is encoded by the DPP7 gene. The protein encoded by this gene is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. The resting lymphocytes are maintained through suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease. The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 81 85 89 85 2.73 3.2% Sample 2 212 226 208 215 215 9.47 4.4% Sample 3 922 965 942 951 945 38.75 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 81 85 89 85 2.73 3.2% Sample 1 86 81 85 89 85 2.73 3.2% Sample 2 212 226 208 215 215 9.47 4.4% Sample 2 212 226 208 215 215 9.47 4.4% Sample 3 922 965 942 951 945 38.75 4.1% Sample 3 922 965 942 951 945 38.75 4.1%

Gene Name

DPP7

Gene ID

29952

UniProt

Q9UHL4

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G22-L492

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 212 922 95 226 892 Standard deviation 4.39 11.87 56.24 3.71 10.85 37.46 CV (%) 5.1% 5.6% 6.1% 3.9% 4.8% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 212 922 95 226 892 Standard deviation 4.39 11.87 56.24 3.71 10.85 37.46 CV (%) 5.1% 5.6% 6.1% 3.9% 4.8% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 86 212 922 95 226 892 Mean (pg/ml) 86 212 922 95 226 892 Standard deviation 4.39 11.87 56.24 3.71 10.85 37.46 Standard deviation 4.39 11.87 56.24 3.71 10.85 37.46 CV (%) 5.1% 5.6% 6.1% 3.9% 4.8% 4.2% CV (%) 5.1% 5.6% 6.1% 3.9% 4.8% 4.2%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Human DPP7

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.12 0.178 0.285 0.507 0.902 1.571 2.684 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.12 0.178 0.285 0.507 0.902 1.571 2.684 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.12 0.178 0.285 0.507 0.902 1.571 2.684 O.D. 0.052 0.12 0.178 0.285 0.507 0.902 1.571 2.684

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.