- Catalog number
- EK2190
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Desmoglein-3 is a protein that in humans is encoded by the DSG3 gene. This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 118 109 112 116 3.82 3.3% Sample 2 268 272 259 266 266 12.51 4.7% Sample 3 652 642 638 674 652 36.48 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 118 109 112 116 3.82 3.3% Sample 1 124 118 109 112 116 3.82 3.3% Sample 2 268 272 259 266 266 12.51 4.7% Sample 2 268 272 259 266 266 12.51 4.7% Sample 3 652 642 638 674 652 36.48 5.6% Sample 3 652 642 638 674 652 36.48 5.6%
- Gene Name
- DSG3
- Gene ID
- 1830
- UniProt
- P32926
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E50-R615
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 268 652 115 279 663 Standard deviation 6.32 9.65 25.43 4.83 13.67 38.45 CV (%) 5.1% 3.6% 3.9% 4.2% 4.9% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 268 652 115 279 663 Standard deviation 6.32 9.65 25.43 4.83 13.67 38.45 CV (%) 5.1% 3.6% 3.9% 4.2% 4.9% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 124 268 652 115 279 663 Mean (pg/ml) 124 268 652 115 279 663 Standard deviation 6.32 9.65 25.43 4.83 13.67 38.45 Standard deviation 6.32 9.65 25.43 4.83 13.67 38.45 CV (%) 5.1% 3.6% 3.9% 4.2% 4.9% 5.8% CV (%) 5.1% 3.6% 3.9% 4.2% 4.9% 5.8%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant Human DSG3
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.043 0.115 0.177 0.301 0.524 0.958 1.644 2.451 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.043 0.115 0.177 0.301 0.524 0.958 1.644 2.451 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.043 0.115 0.177 0.301 0.524 0.958 1.644 2.451 O.D. 0.043 0.115 0.177 0.301 0.524 0.958 1.644 2.451
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
