- Catalog number
- EK2194
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD46 complement regulatory protein also known as CD46 (cluster of differentiation 46) and Membrane Cofactor Protein is a protein which in humans is encoded by the CD46 gene. The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 272 316 294 293 16.38 5.6% Sample 2 1536 1645 1536 1651 1592 105.07 6.6% Sample 3 5471 5741 5863 5716 5698 336.17 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 272 316 294 293 16.38 5.6% Sample 1 288 272 316 294 293 16.38 5.6% Sample 2 1536 1645 1536 1651 1592 105.07 6.6% Sample 2 1536 1645 1536 1651 1592 105.07 6.6% Sample 3 5471 5741 5863 5716 5698 336.17 5.9% Sample 3 5471 5741 5863 5716 5698 336.17 5.9%
- Gene Name
- CD46
- Gene ID
- 4179
- UniProt
- P15529
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: C35-L392
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1536 5471 294 1591 5538 Standard deviation 10.37 59.9 218.84 12.35 57.28 193.83 CV (%) 3.6% 3.9% 4% 4.2% 3.6% 3.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1536 5471 294 1591 5538 Standard deviation 10.37 59.9 218.84 12.35 57.28 193.83 CV (%) 3.6% 3.9% 4% 4.2% 3.6% 3.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 288 1536 5471 294 1591 5538 Mean (pg/ml) 288 1536 5471 294 1591 5538 Standard deviation 10.37 59.9 218.84 12.35 57.28 193.83 Standard deviation 10.37 59.9 218.84 12.35 57.28 193.83 CV (%) 3.6% 3.9% 4% 4.2% 3.6% 3.5% CV (%) 3.6% 3.9% 4% 4.2% 3.6% 3.5%
- Sensitivity
- <20 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant Human CD46
- Sample Data
- Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.009 0.075 0.142 0.265 0.466 0.859 1.528 2.431 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.009 0.075 0.142 0.265 0.466 0.859 1.528 2.431 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.009 0.075 0.142 0.265 0.466 0.859 1.528 2.431 O.D. 0.009 0.075 0.142 0.265 0.466 0.859 1.528 2.431
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
