- Catalog number
- EK2195
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Receptor tyrosine-protein kinase erbB-4 is an enzyme that in humans is encoded by the ERBB4 gene. This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 36 48 59 44 1.81 4.1% Sample 2 149 190 155 162 164 5.9 3.6% Sample 3 868 841 796 822 832 39.09 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 36 48 59 44 1.81 4.1% Sample 1 34 36 48 59 44 1.81 4.1% Sample 2 149 190 155 162 164 5.9 3.6% Sample 2 149 190 155 162 164 5.9 3.6% Sample 3 868 841 796 822 832 39.09 4.7% Sample 3 868 841 796 822 832 39.09 4.7%
- Gene Name
- ERBB4
- Gene ID
- 2066
- UniProt
- Q15303
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q26-R649
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 149 868 46 182 951 Standard deviation 1.5 7.3 37.32 2.58 9.28 52.31 CV (%) 4.4% 4.9% 4.3% 5.6% 5.1% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 149 868 46 182 951 Standard deviation 1.5 7.3 37.32 2.58 9.28 52.31 CV (%) 4.4% 4.9% 4.3% 5.6% 5.1% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 149 868 46 182 951 Mean (pg/ml) 34 149 868 46 182 951 Standard deviation 1.5 7.3 37.32 2.58 9.28 52.31 Standard deviation 1.5 7.3 37.32 2.58 9.28 52.31 CV (%) 4.4% 4.9% 4.3% 5.6% 5.1% 5.5% CV (%) 4.4% 4.9% 4.3% 5.6% 5.1% 5.5%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human ERBB4
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.044 0.131 0.206 0.333 0.55 0.974 1.649 2.725 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.044 0.131 0.206 0.333 0.55 0.974 1.649 2.725 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.044 0.131 0.206 0.333 0.55 0.974 1.649 2.725 O.D. 0.044 0.131 0.206 0.333 0.55 0.974 1.649 2.725
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
