Human FUCA1 ELISA Kit PicoKine®

About this product

Human FUCA1 Kit PicoKine® (96 Tests) . Quantitate Human FUCA1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2203

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tissue alpha-L-fucosidase is an enzyme that in humans is encoded by the FUCA1 gene. The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 243 258 262 287 263 9.45 3.6% Sample 2 486 503 499 512 500 25.50 5.1% Sample 3 937 968 1126 1051 1021 44.9 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 243 258 262 287 263 9.45 3.6% Sample 1 243 258 262 287 263 9.45 3.6% Sample 2 486 503 499 512 500 25.50 5.1% Sample 2 486 503 499 512 500 25.50 5.1% Sample 3 937 968 1126 1051 1021 44.9 4.4% Sample 3 937 968 1126 1051 1021 44.9 4.4%

Gene Name

FUCA1

Gene ID

2517

UniProt

P04066

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q32-K466

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 243 486 937 163 522 1079 Standard deviation 10.94 25.27 59.97 6.36 24.53 66.90 CV (%) 4.5% 5.2% 6.4% 3.9% 4.7% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 243 486 937 163 522 1079 Standard deviation 10.94 25.27 59.97 6.36 24.53 66.90 CV (%) 4.5% 5.2% 6.4% 3.9% 4.7% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 243 486 937 163 522 1079 Mean (pg/ml) 243 486 937 163 522 1079 Standard deviation 10.94 25.27 59.97 6.36 24.53 66.90 Standard deviation 10.94 25.27 59.97 6.36 24.53 66.90 CV (%) 4.5% 5.2% 6.4% 3.9% 4.7% 6.2% CV (%) 4.5% 5.2% 6.4% 3.9% 4.7% 6.2%

Sensitivity

<1 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human FUCA1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.186 0.256 0.419 0.678 1.087 1.587 2.033 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.186 0.256 0.419 0.678 1.087 1.587 2.033 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.186 0.256 0.419 0.678 1.087 1.587 2.033 O.D. 0.084 0.186 0.256 0.419 0.678 1.087 1.587 2.033

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.