Human GGCT/CRF21 ELISA Kit PicoKine®

About this product

Human GGCT/CRF21 Kit PicoKine® (96 Tests) . Quantitate Human GGCT in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2205

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism, and may play a critical role in glutathione homeostasis. The encoded protein may also play a role in cell proliferation, and the expression of this gene is a potential marker for cancer. Pseudogenes of this gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 20. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 181 179 168 202 183 8.76 4.8% Sample 2 452 469 448 486 464 24.58 5.3% Sample 3 841 869 851 883 861 47.36 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 181 179 168 202 183 8.76 4.8% Sample 1 181 179 168 202 183 8.76 4.8% Sample 2 452 469 448 486 464 24.58 5.3% Sample 2 452 469 448 486 464 24.58 5.3% Sample 3 841 869 851 883 861 47.36 5.5% Sample 3 841 869 851 883 861 47.36 5.5%

Gene Name

GGCT

Gene ID

79017

UniProt

O75223

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-L188

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 181 452 841 223 489 892 Standard deviation 9.59 23.05 49.62 10.04 19.07 41.92 CV (%) 5.3% 5.1% 5.9% 4.5% 3.9% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 181 452 841 223 489 892 Standard deviation 9.59 23.05 49.62 10.04 19.07 41.92 CV (%) 5.3% 5.1% 5.9% 4.5% 3.9% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 181 452 841 223 489 892 Mean (pg/ml) 181 452 841 223 489 892 Standard deviation 9.59 23.05 49.62 10.04 19.07 41.92 Standard deviation 9.59 23.05 49.62 10.04 19.07 41.92 CV (%) 5.3% 5.1% 5.9% 4.5% 3.9% 4.7% CV (%) 5.3% 5.1% 5.9% 4.5% 3.9% 4.7%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human GGCT

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.072 0.123 0.217 0.423 0.828 1.55 2.223 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.072 0.123 0.217 0.423 0.828 1.55 2.223 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.072 0.123 0.217 0.423 0.828 1.55 2.223 O.D. 0.009 0.072 0.123 0.217 0.423 0.828 1.55 2.223

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.