Human GLO1 ELISA Kit PicoKine®

About this product

Human GLO1 Kit PicoKine® (96 Tests) . Quantitate Human GLO1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2209

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 350 365 370 368 363 18.53 5.1% Sample 2 1269 1305 1288 1283 1286 46.31 3.6% Sample 3 4565 4541 4720 4690 4629 282.37 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 350 365 370 368 363 18.53 5.1% Sample 1 350 365 370 368 363 18.53 5.1% Sample 2 1269 1305 1288 1283 1286 46.31 3.6% Sample 2 1269 1305 1288 1283 1286 46.31 3.6% Sample 3 4565 4541 4720 4690 4629 282.37 6.1% Sample 3 4565 4541 4720 4690 4629 282.37 6.1%

Gene Name

GLO1

Gene ID

2739

UniProt

Q04760

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-M184

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 350 1269 4565 378 1366 4475 Standard deviation 19.25 65.99 164.34 19.28 61.47 214.8 CV (%) 5.5% 5.2% 3.6% 5.1% 4.5% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 350 1269 4565 378 1366 4475 Standard deviation 19.25 65.99 164.34 19.28 61.47 214.8 CV (%) 5.5% 5.2% 3.6% 5.1% 4.5% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 350 1269 4565 378 1366 4475 Mean (pg/ml) 350 1269 4565 378 1366 4475 Standard deviation 19.25 65.99 164.34 19.28 61.47 214.8 Standard deviation 19.25 65.99 164.34 19.28 61.47 214.8 CV (%) 5.5% 5.2% 3.6% 5.1% 4.5% 4.8% CV (%) 5.5% 5.2% 3.6% 5.1% 4.5% 4.8%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human GLO1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.091 0.141 0.244 0.445 0.813 1.409 2.261 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.091 0.141 0.244 0.445 0.813 1.409 2.261 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.091 0.141 0.244 0.445 0.813 1.409 2.261 O.D. 0.033 0.091 0.141 0.244 0.445 0.813 1.409 2.261

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.