- Catalog number
- EK2216
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- In mammals, histamine is metabolized by two major pathways: N (tau) -methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N (tau) -methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 113 121 118 109 115 4.15 6.1% Sample 2 684 721 706 698 702 42.84 6.1% Sample 3 1463 1522 1469 1463 1479 72.48 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 113 121 118 109 115 4.15 6.1% Sample 1 113 121 118 109 115 4.15 6.1% Sample 2 684 721 706 698 702 42.84 6.1% Sample 2 684 721 706 698 702 42.84 6.1% Sample 3 1463 1522 1469 1463 1479 72.48 4.9% Sample 3 1463 1522 1469 1463 1479 72.48 4.9%
- Gene Name
- HNMT
- Gene ID
- 3176
- UniProt
- P50135
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A2-A292
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 113 684 1463 110 702 1532 Standard deviation 4.41 34.88 70.22 5.72 31.59 78.13 CV (%) 3.9% 5.1% 4.8% 5.2% 4.5% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 113 684 1463 110 702 1532 Standard deviation 4.41 34.88 70.22 5.72 31.59 78.13 CV (%) 3.9% 5.1% 4.8% 5.2% 4.5% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 113 684 1463 110 702 1532 Mean (pg/ml) 113 684 1463 110 702 1532 Standard deviation 4.41 34.88 70.22 5.72 31.59 78.13 Standard deviation 4.41 34.88 70.22 5.72 31.59 78.13 CV (%) 3.9% 5.1% 4.8% 5.2% 4.5% 5.1% CV (%) 3.9% 5.1% 4.8% 5.2% 4.5% 5.1%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human HNMT
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.145 0.231 0.382 0.649 1.038 1.615 1.828 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.145 0.231 0.382 0.649 1.038 1.615 1.828 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.145 0.231 0.382 0.649 1.038 1.615 1.828 O.D. 0.039 0.145 0.231 0.382 0.649 1.038 1.615 1.828
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
