- Catalog number
- EK2218
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Heat shock 10 kDa protein 1 (Hsp10), also known as chaperonin 10 (cpn10) or early-pregnancy factor (EPF), is a protein that in humans is encoded by the HSPE1 gene. This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 112 125 101 112 4.01 3.6% Sample 2 456 496 485 422 465 22.77 4.9% Sample 3 1755 1896 1744 1963 1840 93.81 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 112 125 101 112 4.01 3.6% Sample 1 108 112 125 101 112 4.01 3.6% Sample 2 456 496 485 422 465 22.77 4.9% Sample 2 456 496 485 422 465 22.77 4.9% Sample 3 1755 1896 1744 1963 1840 93.81 5.1% Sample 3 1755 1896 1744 1963 1840 93.81 5.1%
- Gene Name
- HSPE1
- Gene ID
- 3336
- UniProt
- P61604
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: M1-D102
- Capture Antibody
- A polyclonal antibody from goat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 456 1755 115 469 1896 Standard deviation 5.94 22.34 107.06 5.52 25.8 81.53 CV (%) 5.5% 4.9% 6.1% 4.8% 5.5% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 456 1755 115 469 1896 Standard deviation 5.94 22.34 107.06 5.52 25.8 81.53 CV (%) 5.5% 4.9% 6.1% 4.8% 5.5% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 108 456 1755 115 469 1896 Mean (pg/ml) 108 456 1755 115 469 1896 Standard deviation 5.94 22.34 107.06 5.52 25.8 81.53 Standard deviation 5.94 22.34 107.06 5.52 25.8 81.53 CV (%) 5.5% 4.9% 6.1% 4.8% 5.5% 4.3% CV (%) 5.5% 4.9% 6.1% 4.8% 5.5% 4.3%
- Sensitivity
- <75 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human HSPE1
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.08 0.154 0.26 0.504 0.929 1.649 2.48 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.08 0.154 0.26 0.504 0.929 1.649 2.48 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.08 0.154 0.26 0.504 0.929 1.649 2.48 O.D. 0.01 0.08 0.154 0.26 0.504 0.929 1.649 2.48
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
