- Catalog number
- EK2220
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The protein encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biological activities. This enzyme is a type II integral membrane protein and is responsible for 6-O-sulfation of heparan sulfate. This enzyme does not share significant sequence similarity with other known sulfotransferases. A pseudogene located on chromosome 1 has been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 21 18 24 21 0.7 3.3% Sample 2 189 174 163 178 176 7.92 4.5% Sample 3 477 455 436 492 465 27.44 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 21 18 24 21 0.7 3.3% Sample 1 22 21 18 24 21 0.7 3.3% Sample 2 189 174 163 178 176 7.92 4.5% Sample 2 189 174 163 178 176 7.92 4.5% Sample 3 477 455 436 492 465 27.44 5.9% Sample 3 477 455 436 492 465 27.44 5.9%
- Gene Name
- HS6ST1
- Gene ID
- 9394
- UniProt
- O60243
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: P28-W401
- Capture Antibody
- A polyclonal antibody from goat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 189 477 29 176 424 Standard deviation 1.19 12.29 32.91 1.51 9.33 26.29 CV (%) 5.4% 6.5% 6.9% 5.2% 5.3% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 189 477 29 176 424 Standard deviation 1.19 12.29 32.91 1.51 9.33 26.29 CV (%) 5.4% 6.5% 6.9% 5.2% 5.3% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 22 189 477 29 176 424 Mean (pg/ml) 22 189 477 29 176 424 Standard deviation 1.19 12.29 32.91 1.51 9.33 26.29 Standard deviation 1.19 12.29 32.91 1.51 9.33 26.29 CV (%) 5.4% 6.5% 6.9% 5.2% 5.3% 6.2% CV (%) 5.4% 6.5% 6.9% 5.2% 5.3% 6.2%
- Sensitivity
- <7 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human HS6ST1
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.083 0.157 0.279 0.505 0.93 1.55 2.425 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.083 0.157 0.279 0.505 0.93 1.55 2.425 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.083 0.157 0.279 0.505 0.93 1.55 2.425 O.D. 0.011 0.083 0.157 0.279 0.505 0.93 1.55 2.425
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
