Human CD94 ELISA Kit PicoKine®

About this product

Human CD94 PicoKine® ELISA Kit (96 Tests) . Quantitate Human CD94 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 24 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2222

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD94 (Cluster of Differentiation 94), also known as killer cell lectin-like receptor subfamily D, member 1 (KLRD1) is a human gene. Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Several transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 66 80 62 72 2.55 3.6% Sample 2 125 148 150 179 151 10.14 6.7% Sample 3 924 1005 1015 1430 1094 36.96 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 66 80 62 72 2.55 3.6% Sample 1 79 66 80 62 72 2.55 3.6% Sample 2 125 148 150 179 151 10.14 6.7% Sample 2 125 148 150 179 151 10.14 6.7% Sample 3 924 1005 1015 1430 1094 36.96 3.4% Sample 3 924 1005 1015 1430 1094 36.96 3.4%

Gene Name

KLRD1

Gene ID

3824

UniProt

Q13241

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K32-I179

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

46.9 pg/mL - 3,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 172 1200 67 172 1212 Standard deviation 3.29 12.83 87.24 2.3 11.27 63.15 CV (%) 4.2% 7.5% 7.3% 3.4% 6.6% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 172 1200 67 172 1212 Standard deviation 3.29 12.83 87.24 2.3 11.27 63.15 CV (%) 4.2% 7.5% 7.3% 3.4% 6.6% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 78 172 1200 67 172 1212 Mean (pg/ml) 78 172 1200 67 172 1212 Standard deviation 3.29 12.83 87.24 2.3 11.27 63.15 Standard deviation 3.29 12.83 87.24 2.3 11.27 63.15 CV (%) 4.2% 7.5% 7.3% 3.4% 6.6% 5.2% CV (%) 4.2% 7.5% 7.3% 3.4% 6.6% 5.2%

Sensitivity

<24 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human KLRD1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.038 0.109 0.184 0.306 0.532 0.957 1.637 2.508 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.038 0.109 0.184 0.306 0.532 0.957 1.637 2.508 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.038 0.109 0.184 0.306 0.532 0.957 1.637 2.508 O.D. 0.038 0.109 0.184 0.306 0.532 0.957 1.637 2.508

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.