Human Jagged1 ELISA Kit PicoKine®

About this product

Human Jagged1 PicoKine® ELISA Kit (96 Tests) . Quantitate Human Jagged1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 30 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2224

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter is involved in signaling processes. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 96 75 67 82 4.44 5.4% Sample 2 185 143 239 169 184 12.6 6.9% Sample 3 1704 1270 1116 1693 1446 93.40 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 96 75 67 82 4.44 5.4% Sample 1 90 96 75 67 82 4.44 5.4% Sample 2 185 143 239 169 184 12.6 6.9% Sample 2 185 143 239 169 184 12.6 6.9% Sample 3 1704 1270 1116 1693 1446 93.40 6.5% Sample 3 1704 1270 1116 1693 1446 93.40 6.5%

Gene Name

JAG1

Gene ID

182

UniProt

P78504

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: S32-S1046

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 185 1704 76 200 1687 Standard deviation 2.79 12.91 48.39 4.92 10.12 69.34 CV (%) 3.1% 7% 2.8% 6.5% 5.1% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 185 1704 76 200 1687 Standard deviation 2.79 12.91 48.39 4.92 10.12 69.34 CV (%) 3.1% 7% 2.8% 6.5% 5.1% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 185 1704 76 200 1687 Mean (pg/ml) 90 185 1704 76 200 1687 Standard deviation 2.79 12.91 48.39 4.92 10.12 69.34 Standard deviation 2.79 12.91 48.39 4.92 10.12 69.34 CV (%) 3.1% 7% 2.8% 6.5% 5.1% 4.1% CV (%) 3.1% 7% 2.8% 6.5% 5.1% 4.1%

Sensitivity

<30 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human JAG1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.085 0.117 0.19 0.333 0.592 1.04 1.785 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.085 0.117 0.19 0.333 0.592 1.04 1.785 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.085 0.117 0.19 0.333 0.592 1.04 1.785 O.D. 0.032 0.085 0.117 0.19 0.333 0.592 1.04 1.785

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.