Human Intergrin Alpha 5/CD49E ELISA Kit PicoKine®

About this product

Human Intergrin Alpha 5/CD49E PicoKine® ELISA Kit (96 Tests) . Quantitate Human Intergrin Alpha 5/CD49E in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2227

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Integrin alpha-5 is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 166 180 215 201 9.23 4.6% Sample 2 502 527 467 385 470 32.92 7% Sample 3 2865 4075 4582 2563 3521 196.49 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 166 180 215 201 9.23 4.6% Sample 1 242 166 180 215 201 9.23 4.6% Sample 2 502 527 467 385 470 32.92 7% Sample 2 502 527 467 385 470 32.92 7% Sample 3 2865 4075 4582 2563 3521 196.49 5.6% Sample 3 2865 4075 4582 2563 3521 196.49 5.6%

Gene Name

ITGA5

Gene ID

3678

UniProt

P08648

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F42-Y995

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 502 2865 207 579 4142 Standard deviation 18.27 39.11 115.46 13.50 32.48 277.93 CV (%) 7.6% 7.8% 4% 6.5% 5.6% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 502 2865 207 579 4142 Standard deviation 18.27 39.11 115.46 13.50 32.48 277.93 CV (%) 7.6% 7.8% 4% 6.5% 5.6% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 242 502 2865 207 579 4142 Mean (pg/ml) 242 502 2865 207 579 4142 Standard deviation 18.27 39.11 115.46 13.50 32.48 277.93 Standard deviation 18.27 39.11 115.46 13.50 32.48 277.93 CV (%) 7.6% 7.8% 4% 6.5% 5.6% 6.7% CV (%) 7.6% 7.8% 4% 6.5% 5.6% 6.7%

Sensitivity

<75 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ITGA5

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.093 0.164 0.291 0.551 0.944 1.568 2.139 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.093 0.164 0.291 0.551 0.944 1.568 2.139 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.093 0.164 0.291 0.551 0.944 1.568 2.139 O.D. 0.015 0.093 0.164 0.291 0.551 0.944 1.568 2.139

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.