Human LRG1 ELISA Kit PicoKine®

About this product

Human LRG1 PicoKine® ELISA Kit (96 Tests) . Quantitate Human LRG1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 300 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2233

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Leucine-rich alpha-2-glycoprotein 1 is a protein which in humans is encoded by the gene LRG1. The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1060 820 1217 1160 1064 60.77 5.7% Sample 2 2321 1411 1653 1516 1725 113.18 6.6% Sample 3 18753 15634 16014 17741 17036 1333.88 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1060 820 1217 1160 1064 60.77 5.7% Sample 1 1060 820 1217 1160 1064 60.77 5.7% Sample 2 2321 1411 1653 1516 1725 113.18 6.6% Sample 2 2321 1411 1653 1516 1725 113.18 6.6% Sample 3 18753 15634 16014 17741 17036 1333.88 7.8% Sample 3 18753 15634 16014 17741 17036 1333.88 7.8%

Gene Name

LRG1

Gene ID

116844

UniProt

P02750

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: HEK293; Immunogen sequence: V36-Q347

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1060 2321 18753 626 1268 12203 Standard deviation 42.29 118.84 1177.69 14.27 91.42 541.81 CV (%) 4% 5.1% 6.3% 2.3% 7.2% 4.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1060 2321 18753 626 1268 12203 Standard deviation 42.29 118.84 1177.69 14.27 91.42 541.81 CV (%) 4% 5.1% 6.3% 2.3% 7.2% 4.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1060 2321 18753 626 1268 12203 Mean (pg/ml) 1060 2321 18753 626 1268 12203 Standard deviation 42.29 118.84 1177.69 14.27 91.42 541.81 Standard deviation 42.29 118.84 1177.69 14.27 91.42 541.81 CV (%) 4% 5.1% 6.3% 2.3% 7.2% 4.4% CV (%) 4% 5.1% 6.3% 2.3% 7.2% 4.4%

Sensitivity

<300 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human LRG1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.