Human LECT2 ELISA Kit PicoKine®

About this product

Human LECT2 PicoKine® ELISA Kit (96 Tests) . Quantitate Human LECT2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 15 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2235

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a secreted, 16 kDa protein that acts as a chemotactic factor to neutrophils and stimulates the growth of chondrocytes and osteoblasts. This protein has high sequence similarity to the chondromodulin repeat regions of the chicken myb-induced myeloid 1 protein. A polymorphism in this gene may be associated with rheumatoid arthritis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 38 52 45 42 2.22 5.3% Sample 2 98 79 106 108 98 3.76 3.9% Sample 3 966 728 930 937 890 51.46 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 38 52 45 42 2.22 5.3% Sample 1 32 38 52 45 42 2.22 5.3% Sample 2 98 79 106 108 98 3.76 3.9% Sample 2 98 79 106 108 98 3.76 3.9% Sample 3 966 728 930 937 890 51.46 5.8% Sample 3 966 728 930 937 890 51.46 5.8%

Gene Name

LECT2

Gene ID

3950

UniProt

O14960

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: HEK293; Immunogen sequence: G19-L151

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 98 966 53 69 748 Standard deviation 1.75 5.44 48.49 2.01 4.33 42.04 CV (%) 5.5% 5.6% 5% 3.8% 6.3% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 98 966 53 69 748 Standard deviation 1.75 5.44 48.49 2.01 4.33 42.04 CV (%) 5.5% 5.6% 5% 3.8% 6.3% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 98 966 53 69 748 Mean (pg/ml) 32 98 966 53 69 748 Standard deviation 1.75 5.44 48.49 2.01 4.33 42.04 Standard deviation 1.75 5.44 48.49 2.01 4.33 42.04 CV (%) 5.5% 5.6% 5% 3.8% 6.3% 5.6% CV (%) 5.5% 5.6% 5% 3.8% 6.3% 5.6%

Sensitivity

<15 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human LECT2

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.22 0.314 0.494 0.757 1.205 1.839 2.828 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.22 0.314 0.494 0.757 1.205 1.839 2.828 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.22 0.314 0.494 0.757 1.205 1.839 2.828 O.D. 0.063 0.22 0.314 0.494 0.757 1.205 1.839 2.828

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.