- Catalog number
- EK0865
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23) . IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B) . The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG) . In contrast to IL12, which acts mainly on naive CD4 (+) T cells, IL23 preferentially acts on memory CD4 (+) T cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 280 157 260 211 227 10.62 4.7% Sample 2 519 602 510 606 559 33.22 5.9% Sample 3 4052 4755 3861 3421 4022 259.84 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 280 157 260 211 227 10.62 4.7% Sample 1 280 157 260 211 227 10.62 4.7% Sample 2 519 602 510 606 559 33.22 5.9% Sample 2 519 602 510 606 559 33.22 5.9% Sample 3 4052 4755 3861 3421 4022 259.84 6.5% Sample 3 4052 4755 3861 3421 4022 259.84 6.5%
- Gene Name
- IL23A
- Gene ID
- 51561
- UniProt
- Q9NPF7
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: (R20-P189) + (I23-S328)
- Capture Antibody
- A polyclonal antibody from goat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 280 519 4052 195 572 2736 Standard deviation 17.22 14.12 142.63 8.09 15.44 98.5 CV (%) 6.2% 2.7% 3.5% 4.2% 2.7% 3.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 280 519 4052 195 572 2736 Standard deviation 17.22 14.12 142.63 8.09 15.44 98.5 CV (%) 6.2% 2.7% 3.5% 4.2% 2.7% 3.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 280 519 4052 195 572 2736 Mean (pg/ml) 280 519 4052 195 572 2736 Standard deviation 17.22 14.12 142.63 8.09 15.44 98.5 Standard deviation 17.22 14.12 142.63 8.09 15.44 98.5 CV (%) 6.2% 2.7% 3.5% 4.2% 2.7% 3.6% CV (%) 6.2% 2.7% 3.5% 4.2% 2.7% 3.6%
- Sensitivity
- <75 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human IL23A
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.11 0.203 0.348 0.568 0.975 1.495 2.001 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.11 0.203 0.348 0.568 0.975 1.495 2.001 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.11 0.203 0.348 0.568 0.975 1.495 2.001 O.D. 0.009 0.11 0.203 0.348 0.568 0.975 1.495 2.001
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
