- Catalog number
- EK2242
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Macrophage receptor with collagenous structure (MARCO) is a protein that in humans is encoded by the MARCO gene. The protein encoded by this gene is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 256 289 206 239 10.49 4.4% Sample 2 948 1068 823 1021 965 45.55 4.7% Sample 3 2703 4988 3405 2885 3495 114.29 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 256 289 206 239 10.49 4.4% Sample 1 203 256 289 206 239 10.49 4.4% Sample 2 948 1068 823 1021 965 45.55 4.7% Sample 2 948 1068 823 1021 965 45.55 4.7% Sample 3 2703 4988 3405 2885 3495 114.29 3.3% Sample 3 2703 4988 3405 2885 3495 114.29 3.3%
- Gene Name
- MARCO
- Gene ID
- 8685
- UniProt
- Q9UEW3
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: M79-V520
- Capture Antibody
- A polyclonal antibody from goat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 948 2703 303 878 3050 Standard deviation 6.56 34.51 132.18 14.15 47.32 219.6 CV (%) 3.2% 3.6% 4.9% 4.7% 5.4% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 948 2703 303 878 3050 Standard deviation 6.56 34.51 132.18 14.15 47.32 219.6 CV (%) 3.2% 3.6% 4.9% 4.7% 5.4% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 203 948 2703 303 878 3050 Mean (pg/ml) 203 948 2703 303 878 3050 Standard deviation 6.56 34.51 132.18 14.15 47.32 219.6 Standard deviation 6.56 34.51 132.18 14.15 47.32 219.6 CV (%) 3.2% 3.6% 4.9% 4.7% 5.4% 7.2% CV (%) 3.2% 3.6% 4.9% 4.7% 5.4% 7.2%
- Sensitivity
- <150 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human MARCO
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 2.061 2.564 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 2.061 2.564 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 2.061 2.564 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 2.061 2.564
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
