Human MYDGF ELISA Kit PicoKine®

About this product

Human MYDGF PicoKine® ELISA Kit (96 Tests) . Quantitate Human MYDGF in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2253

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 262 246 247 282 259 13.58 5.2% Sample 2 531 428 355 520 459 27.6 6% Sample 3 4821 4582 4248 4528 4545 345.58 7.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 262 246 247 282 259 13.58 5.2% Sample 1 262 246 247 282 259 13.58 5.2% Sample 2 531 428 355 520 459 27.6 6% Sample 2 531 428 355 520 459 27.6 6% Sample 3 4821 4582 4248 4528 4545 345.58 7.6% Sample 3 4821 4582 4248 4528 4545 345.58 7.6%

Gene Name

MYDGF

Gene ID

56005

UniProt

Q969H8

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: V32-L173

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 262 531 4821 222 447 4891 Standard deviation 11.14 26.39 300.35 14.27 10.77 375.63 CV (%) 4.3% 5.0% 6.2% 6.4% 2.4% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 262 531 4821 222 447 4891 Standard deviation 11.14 26.39 300.35 14.27 10.77 375.63 CV (%) 4.3% 5.0% 6.2% 6.4% 2.4% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 262 531 4821 222 447 4891 Mean (pg/ml) 262 531 4821 222 447 4891 Standard deviation 11.14 26.39 300.35 14.27 10.77 375.63 Standard deviation 11.14 26.39 300.35 14.27 10.77 375.63 CV (%) 4.3% 5.0% 6.2% 6.4% 2.4% 7.7% CV (%) 4.3% 5.0% 6.2% 6.4% 2.4% 7.7%

Sensitivity

<75 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human MYDGF

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.117 0.198 0.338 0.625 1.098 1.804 2.384 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.117 0.198 0.338 0.625 1.098 1.804 2.384 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.117 0.198 0.338 0.625 1.098 1.804 2.384 O.D. 0.057 0.117 0.198 0.338 0.625 1.098 1.804 2.384

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.