- Catalog number
- EK2256
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Paired immunoglobulin-like type 2 receptor beta is a protein that in humans is encoded by the PILRB gene. The paired immunoglobin-like type 2 receptors consist of highly related activating and inhibitory receptors that are involved in the regulation of many aspects of the immune system. The paired immunoglobulin-like receptor genes are located in a tandem head-to-tail orientation on chromosome 7. This gene encodes the activating member of the receptor pair and contains a truncated cytoplasmic tail relative to its inhibitory counterpart (PILRA), that has a long cytoplasmic tail with immunoreceptor tyrosine-based inhibitory (ITIM) motifs. This gene is thought to have arisen from a duplication of the inhibitory PILRA gene and evolved to acquire its activating function.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 101 134 148 125 9.16 7.3% Sample 2 321 303 263 351 310 19.07 6.2% Sample 3 2663 1678 2544 1933 2205 141.31 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 101 134 148 125 9.16 7.3% Sample 1 116 101 134 148 125 9.16 7.3% Sample 2 321 303 263 351 310 19.07 6.2% Sample 2 321 303 263 351 310 19.07 6.2% Sample 3 2663 1678 2544 1933 2205 141.31 6.4% Sample 3 2663 1678 2544 1933 2205 141.31 6.4%
- Gene Name
- PILRB
- Gene ID
- 29990
- UniProt
- Q9UKJ0
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: HEK293; Immunogen sequence: Q20-A189
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 93.7 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 321 2663 143 371 2601 Standard deviation 9.2 16.15 136.88 3.47 9.35 99.62 CV (%) 7.9% 5% 5.1% 2.4% 2.5% 3.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 321 2663 143 371 2601 Standard deviation 9.2 16.15 136.88 3.47 9.35 99.62 CV (%) 7.9% 5% 5.1% 2.4% 2.5% 3.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 116 321 2663 143 371 2601 Mean (pg/ml) 116 321 2663 143 371 2601 Standard deviation 9.2 16.15 136.88 3.47 9.35 99.62 Standard deviation 9.2 16.15 136.88 3.47 9.35 99.62 CV (%) 7.9% 5% 5.1% 2.4% 2.5% 3.8% CV (%) 7.9% 5% 5.1% 2.4% 2.5% 3.8%
- Sensitivity
- <45 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human PILRB
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.015 0.079 0.148 0.283 0.583 0.992 1.632 1.961 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.015 0.079 0.148 0.283 0.583 0.992 1.632 1.961 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.015 0.079 0.148 0.283 0.583 0.992 1.632 1.961 O.D. 0.015 0.079 0.148 0.283 0.583 0.992 1.632 1.961
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
