- Catalog number
- EK2257
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Pyruvate kinase PKLR is an enzyme that in humans is encoded by the PKLR gene. The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA) . Multiple transcript variants encoding different isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 168 186 177 179 13.75 7.7% Sample 2 335 263 278 311 297 11.07 3.7% Sample 3 2366 1875 2286 2162 2172 144.02 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 168 186 177 179 13.75 7.7% Sample 1 185 168 186 177 179 13.75 7.7% Sample 2 335 263 278 311 297 11.07 3.7% Sample 2 335 263 278 311 297 11.07 3.7% Sample 3 2366 1875 2286 2162 2172 144.02 6.6% Sample 3 2366 1875 2286 2162 2172 144.02 6.6%
- Gene Name
- PKLR
- Gene ID
- 5313
- UniProt
- P30613
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: L47-S574
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
- Detection Range
- 93.7 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 335 2366 185 267 1851 Standard deviation 7.25 13.97 57.97 8.94 18.42 116.8 CV (%) 3.9% 4.2% 2.5% 4.8% 6.9% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 335 2366 185 267 1851 Standard deviation 7.25 13.97 57.97 8.94 18.42 116.8 CV (%) 3.9% 4.2% 2.5% 4.8% 6.9% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 185 335 2366 185 267 1851 Mean (pg/ml) 185 335 2366 185 267 1851 Standard deviation 7.25 13.97 57.97 8.94 18.42 116.8 Standard deviation 7.25 13.97 57.97 8.94 18.42 116.8 CV (%) 3.9% 4.2% 2.5% 4.8% 6.9% 6.3% CV (%) 3.9% 4.2% 2.5% 4.8% 6.9% 6.3%
- Sensitivity
- <45 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human PKLR
- Anticoagulant
- Heparin, EDTA or citrate
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.026 0.116 0.197 0.338 0.557 1.066 1.565 2.056 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.026 0.116 0.197 0.338 0.557 1.066 1.565 2.056 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.026 0.116 0.197 0.338 0.557 1.066 1.565 2.056 O.D. 0.026 0.116 0.197 0.338 0.557 1.066 1.565 2.056
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
