Human OSCAR ELISA Kit PicoKine®

About this product

Human OSCAR ELISA Kit PicoKine® (96 Tests) . Quantitate Human OSCAR in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2270

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Osteoclasts are multinucleated cells that resorb bone and are essential for bone homeostasis. This gene encodes an osteoclast-associated receptor (OSCAR), which is a member of the leukocyte receptor complex protein family that plays critical roles in the regulation of both innate and adaptive immune responses. The encoded protein may play a role in oxidative stress-mediated atherogenesis as well as monocyte adhesion. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 233 204 263 232 233 15.52 6.7% Sample 2 463 406 625 332 457 10.09 2.2% Sample 3 4178 2680 3831 3264 3488 223.25 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 233 204 263 232 233 15.52 6.7% Sample 1 233 204 263 232 233 15.52 6.7% Sample 2 463 406 625 332 457 10.09 2.2% Sample 2 463 406 625 332 457 10.09 2.2% Sample 3 4178 2680 3831 3264 3488 223.25 6.4% Sample 3 4178 2680 3831 3264 3488 223.25 6.4%

Gene Name

OSCAR

Gene ID

126014

UniProt

Q8IYS5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-F219

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 233 463 4178 165 552 4061 Standard deviation 10.42 33.61 166.57 9.62 37.81 189.24 CV (%) 4.5% 7.3% 2.8% 5.8% 6.9% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 233 463 4178 165 552 4061 Standard deviation 10.42 33.61 166.57 9.62 37.81 189.24 CV (%) 4.5% 7.3% 2.8% 5.8% 6.9% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 233 463 4178 165 552 4061 Mean (pg/ml) 233 463 4178 165 552 4061 Standard deviation 10.42 33.61 166.57 9.62 37.81 189.24 Standard deviation 10.42 33.61 166.57 9.62 37.81 189.24 CV (%) 4.5% 7.3% 2.8% 5.8% 6.9% 4.7% CV (%) 4.5% 7.3% 2.8% 5.8% 6.9% 4.7%

Sensitivity

<75 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human OSCAR

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.181 0.304 0.47 0.775 1.228 1.704 2.065 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.181 0.304 0.47 0.775 1.228 1.704 2.065 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.181 0.304 0.47 0.775 1.228 1.704 2.065 O.D. 0.062 0.181 0.304 0.47 0.775 1.228 1.704 2.065

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.