Human Nidogen2 ELISA Kit PicoKine®

About this product

Human Nidogen2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human Nidogen2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2273

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a member of the nidogen family of basement membrane proteins. This protein is a cell-adhesion protein that binds collagens I and IV and laminin and may be involved in maintaining the structure of the basement membrane.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 217 187 287 163 214 5.64 2.6% Sample 2 475 399 511 593 495 37.19 7.5% Sample 3 3201 4223 3220 4079 3681 237.04 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 217 187 287 163 214 5.64 2.6% Sample 1 217 187 287 163 214 5.64 2.6% Sample 2 475 399 511 593 495 37.19 7.5% Sample 2 475 399 511 593 495 37.19 7.5% Sample 3 3201 4223 3220 4079 3681 237.04 6.4% Sample 3 3201 4223 3220 4079 3681 237.04 6.4%

Gene Name

NID2

Gene ID

22795

UniProt

Q14112

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: L31-K1375

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 217 475 3201 191 346 3527 Standard deviation 16.14 24.61 114.28 11.56 14.81 232.78 CV (%) 7.4% 5.2% 3.6% 6.1% 4.3% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 217 475 3201 191 346 3527 Standard deviation 16.14 24.61 114.28 11.56 14.81 232.78 CV (%) 7.4% 5.2% 3.6% 6.1% 4.3% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 217 475 3201 191 346 3527 Mean (pg/ml) 217 475 3201 191 346 3527 Standard deviation 16.14 24.61 114.28 11.56 14.81 232.78 Standard deviation 16.14 24.61 114.28 11.56 14.81 232.78 CV (%) 7.4% 5.2% 3.6% 6.1% 4.3% 6.6% CV (%) 7.4% 5.2% 3.6% 6.1% 4.3% 6.6%

Sensitivity

<75 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human NID2

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.178 0.292 0.456 0.78 1.215 1.737 2.068 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.178 0.292 0.456 0.78 1.215 1.737 2.068 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.178 0.292 0.456 0.78 1.215 1.737 2.068 O.D. 0.062 0.178 0.292 0.456 0.78 1.215 1.737 2.068

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.