- Catalog number
- EK2279
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Matrix extracellular phosphoglycoprotein (Osteoblast/osteocyte factor 45) is a protein that in humans is encoded by the MEPE gene. This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 224 226 307 234 248 18.01 7.3% Sample 2 462 335 340 457 399 19.57 4.9% Sample 3 4230 4343 3648 4613 4209 247.88 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 224 226 307 234 248 18.01 7.3% Sample 1 224 226 307 234 248 18.01 7.3% Sample 2 462 335 340 457 399 19.57 4.9% Sample 2 462 335 340 457 399 19.57 4.9% Sample 3 4230 4343 3648 4613 4209 247.88 5.9% Sample 3 4230 4343 3648 4613 4209 247.88 5.9%
- Gene Name
- MEPE
- Gene ID
- 56955
- UniProt
- Q9NQ76
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: P18-D525
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 224 462 4230 205 609 3785 Standard deviation 15.39 18.94 157.78 15.95 19.37 123.39 CV (%) 6.9% 4.1% 3.7% 7.8% 3.2% 3.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 224 462 4230 205 609 3785 Standard deviation 15.39 18.94 157.78 15.95 19.37 123.39 CV (%) 6.9% 4.1% 3.7% 7.8% 3.2% 3.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 224 462 4230 205 609 3785 Mean (pg/ml) 224 462 4230 205 609 3785 Standard deviation 15.39 18.94 157.78 15.95 19.37 123.39 Standard deviation 15.39 18.94 157.78 15.95 19.37 123.39 CV (%) 6.9% 4.1% 3.7% 7.8% 3.2% 3.3% CV (%) 6.9% 4.1% 3.7% 7.8% 3.2% 3.3%
- Sensitivity
- <75 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human MEPE
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.173 0.288 0.454 0.765 1.204 1.674 2.046 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.173 0.288 0.454 0.765 1.204 1.674 2.046 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.173 0.288 0.454 0.765 1.204 1.674 2.046 O.D. 0.049 0.173 0.288 0.454 0.765 1.204 1.674 2.046
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
