Mouse OSCAR ELISA Kit PicoKine®

About this product

Mouse OSCAR ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse OSCAR in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2281

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Osteoclasts are multinucleated cells that resorb bone and are essential for bone homeostasis. This gene encodes an osteoclast-associated receptor (OSCAR), which is a member of the leukocyte receptor complex protein family that plays critical roles in the regulation of both innate and adaptive immune responses. The encoded protein may play a role in oxidative stress-mediated atherogenesis as well as monocyte adhesion. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 85 104 86 140 104 4.59 4.4% Sample 2 218 289 184 163 214 14.43 6.8% Sample 3 2107 2156 1988 1334 1896 41.53 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 85 104 86 140 104 4.59 4.4% Sample 1 85 104 86 140 104 4.59 4.4% Sample 2 218 289 184 163 214 14.43 6.8% Sample 2 218 289 184 163 214 14.43 6.8% Sample 3 2107 2156 1988 1334 1896 41.53 2.2% Sample 3 2107 2156 1988 1334 1896 41.53 2.2%

Gene Name

Oscar

Gene ID

232790

UniProt

Q8VBT3

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D19-N228

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 218 2107 108 167 2056 Standard deviation 2.11 7.56 107.04 4.27 4.63 134.67 CV (%) 2.5% 3.5% 5.1% 4% 2.8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 218 2107 108 167 2056 Standard deviation 2.11 7.56 107.04 4.27 4.63 134.67 CV (%) 2.5% 3.5% 5.1% 4% 2.8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 85 218 2107 108 167 2056 Mean (pg/ml) 85 218 2107 108 167 2056 Standard deviation 2.11 7.56 107.04 4.27 4.63 134.67 Standard deviation 2.11 7.56 107.04 4.27 4.63 134.67 CV (%) 2.5% 3.5% 5.1% 4% 2.8% 6.6% CV (%) 2.5% 3.5% 5.1% 4% 2.8% 6.6%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human LY96

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.023 0.072 0.137 0.269 0.584 0.968 1.769 2.851 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.023 0.072 0.137 0.269 0.584 0.968 1.769 2.851 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.023 0.072 0.137 0.269 0.584 0.968 1.769 2.851 O.D. 0.023 0.072 0.137 0.269 0.584 0.968 1.769 2.851

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.