- Catalog number
- EK2288
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- C-Peptide, or connecting peptide, is a short peptide chain that is produced during the synthesis of insulin. When proinsulin is cleaved to form insulin, C-Peptide is released as a byproduct. It serves as a useful biomarker for assessing insulin production in the body because its levels reflect the amount of insulin secreted by the pancreas. Unlike insulin, C-Peptide has a longer half-life in the bloodstream, making it easier to measure. Elevated levels of C-Peptide can indicate insulinoma or insulin resistance, while low levels may suggest type 1 diabetes or pancreatic dysfunction. Understanding C-Peptide levels can help in diagnosing and managing various metabolic disorders.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 72 106 71 80 5.23 6.6% Sample 2 318 500 455 447 430 15.01 3.5% Sample 3 1720 1706 1260 1331 1504 117.63 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 72 106 71 80 5.23 6.6% Sample 1 69 72 106 71 80 5.23 6.6% Sample 2 318 500 455 447 430 15.01 3.5% Sample 2 318 500 455 447 430 15.01 3.5% Sample 3 1720 1706 1260 1331 1504 117.63 7.8% Sample 3 1720 1706 1260 1331 1504 117.63 7.8%
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: human peptide
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 2 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 318 1720 92 351 1533 Standard deviation 1.6 17.84 50.05 4.66 13.3 66.84 CV (%) 2.3% 5.6% 2.9% 5.1% 3.8% 4.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 318 1720 92 351 1533 Standard deviation 1.6 17.84 50.05 4.66 13.3 66.84 CV (%) 2.3% 5.6% 2.9% 5.1% 3.8% 4.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 69 318 1720 92 351 1533 Mean (pg/ml) 69 318 1720 92 351 1533 Standard deviation 1.6 17.84 50.05 4.66 13.3 66.84 Standard deviation 1.6 17.84 50.05 4.66 13.3 66.84 CV (%) 2.3% 5.6% 2.9% 5.1% 3.8% 4.4% CV (%) 2.3% 5.6% 2.9% 5.1% 3.8% 4.4%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|5
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.122 0.21 0.368 0.654 1.089 1.651 2.237 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.122 0.21 0.368 0.654 1.089 1.651 2.237 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.122 0.21 0.368 0.654 1.089 1.651 2.237 O.D. 0.045 0.122 0.21 0.368 0.654 1.089 1.651 2.237
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
