Human IL-12 (p70) PicoKine® Quick ELISA Kit

About this product

Human IL-12 (P70) PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate human IL12A in cell culture supernatants and serum. Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0421

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin (IL) -12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response.[i] And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.[ii] Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.[iii] The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 20 17 18 1.08 6% Sample 2 51 59 60 54 56 3.67 6.5% Sample 3 294 290 284 296 291 4.58 1.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 20 17 18 1.08 6% Sample 1 18 18 20 17 18 1.08 6% Sample 2 51 59 60 54 56 3.67 6.5% Sample 2 51 59 60 54 56 3.67 6.5% Sample 3 294 290 284 296 291 4.58 1.5% Sample 3 294 290 284 296 291 4.58 1.5%

Gene Name

IL12A

Gene ID

3592

UniProt

P29459

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: (I23-S328) + (R23-S219)

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

7.8 pg/mL - 500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 51 294 19 55 310 Standard deviation 1.31 3.41 17.38 1.67 3.79 21.39 CV (%) 7.3% 6.7% 5.2% 8.8% 6.9% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 51 294 19 55 310 Standard deviation 1.31 3.41 17.38 1.67 3.79 21.39 CV (%) 7.3% 6.7% 5.2% 8.8% 6.9% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 51 294 19 55 310 Mean (pg/ml) 18 51 294 19 55 310 Standard deviation 1.31 3.41 17.38 1.67 3.79 21.39 Standard deviation 1.31 3.41 17.38 1.67 3.79 21.39 CV (%) 7.3% 6.7% 5.2% 8.8% 6.9% 6.9% CV (%) 7.3% 6.7% 5.2% 8.8% 6.9% 6.9%

Sensitivity

<2 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.021 0.088 0.140 0.274 0.513 0.953 1.620 2.044 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.021 0.088 0.140 0.274 0.513 0.953 1.620 2.044 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.021 0.088 0.140 0.274 0.513 0.953 1.620 2.044 O.D. 0.021 0.088 0.140 0.274 0.513 0.953 1.620 2.044

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.