Human MMP-2 PicoKine® Quick ELISA Kit

About this product

Human MMP-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate human MMP-2 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0459

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 952 1166 1130 693 985 50.15 5.1% Sample 2 1335 2149 1773 1456 1668 39.37 2.4% Sample 3 18623 16083 10251 14876 14868 793.96 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 952 1166 1130 693 985 50.15 5.1% Sample 1 952 1166 1130 693 985 50.15 5.1% Sample 2 1335 2149 1773 1456 1668 39.37 2.4% Sample 2 1335 2149 1773 1456 1668 39.37 2.4% Sample 3 18623 16083 10251 14876 14868 793.96 5.3% Sample 3 18623 16083 10251 14876 14868 793.96 5.3%

Gene Name

INHBA

Gene ID

3624

UniProt

Q15389

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: G311-S426

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 952 1335 18263 716 1356 10353 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 952 1335 18263 716 1356 10353 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 952 1335 18263 716 1356 10353 Mean (pg/ml) 952 1335 18263 716 1356 10353 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7% CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Anticoagulant

Heparin

Sample Data

Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 O.D. 0.015 0.190 0.335 0.627 1.138 1.738 1.905 2.079 Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 O.D. 0.015 0.190 0.335 0.627 1.138 1.738 1.905 2.079 Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 O.D. 0.015 0.190 0.335 0.627 1.138 1.738 1.905 2.079 O.D. 0.015 0.190 0.335 0.627 1.138 1.738 1.905 2.079

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.