- Catalog number
- FEK1295
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 311 293 312 282 299 12.61 4.2% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 311 293 312 282 299 12.61 4.2% Sample 1 311 293 312 282 299 12.61 4.2% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3%
- Gene Name
- ANGPT1
- Gene ID
- 89807
- UniProt
- O35460
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: H16-F498
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.084 0.147 0.222 0.338 0.568 1.017 1.673 2.044 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.084 0.147 0.222 0.338 0.568 1.017 1.673 2.044 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.084 0.147 0.222 0.338 0.568 1.017 1.673 2.044 O.D. 0.084 0.147 0.222 0.338 0.568 1.017 1.673 2.044
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
