Mouse IGF-2 PicoKine® Quick ELISA Kit

About this product

Mouse IGF-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Igf2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0381

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 84 91 92 87 4.03 4.6% Sample 2 719 780 698 710 726 31.63 4.3% Sample 3 980 989 1144 1081 1048 67.83 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 84 91 92 87 4.03 4.6% Sample 1 83 84 91 92 87 4.03 4.6% Sample 2 719 780 698 710 726 31.63 4.3% Sample 2 719 780 698 710 726 31.63 4.3% Sample 3 980 989 1144 1081 1048 67.83 6.4% Sample 3 980 989 1144 1081 1048 67.83 6.4%

Synonyms

Insulin-like growth factor II; IGF-II; Multiplication-stimulating polypeptide; Insulin-like growth factor II; Preptin; Igf2; Igf-2

Gene Name

IGF2

Gene ID

16002

UniProt

P09535

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A25-E91

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 719 980 84 692 946 Standard deviation 5.89 56.08 61.74 6.55 63.66 73.78 CV (%) 7.1% 7.8% 9.2% 7.8% 9.2% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 719 980 84 692 946 Standard deviation 5.89 56.08 61.74 6.55 63.66 73.78 CV (%) 7.1% 7.8% 9.2% 7.8% 9.2% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 83 719 980 84 692 946 Mean (pg/ml) 83 719 980 84 692 946 Standard deviation 5.89 56.08 61.74 6.55 63.66 73.78 Standard deviation 5.89 56.08 61.74 6.55 63.66 73.78 CV (%) 7.1% 7.8% 9.2% 7.8% 9.2% 7.8% CV (%) 7.1% 7.8% 9.2% 7.8% 9.2% 7.8%

Sensitivity

<5 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20030 MW

Specificity

Natural and recombinant mouse IGF-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Insulin-like growth factor II

Protein Name

Insulin-like growth factor II

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.065 0.133 0.190 0.316 0.589 1.117 1.885 2.032 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.065 0.133 0.190 0.316 0.589 1.117 1.885 2.032 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.065 0.133 0.190 0.316 0.589 1.117 1.885 2.032 O.D. 0.065 0.133 0.190 0.316 0.589 1.117 1.885 2.032

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.