Human ACE PicoKine® Quick ELISA Kit

About this product

Human ACE/Cd143 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human ACE in cell culture supernatants, serum, plasma (heparin) and saliva. Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0557

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1278 1310 1292 1425 1326 58.13 4.3% Sample 2 6839 6994 7039 6669 6885 145.23 2.1% Sample 3 24157 24884 22207 21600 23212 1350.66 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1278 1310 1292 1425 1326 58.13 4.3% Sample 1 1278 1310 1292 1425 1326 58.13 4.3% Sample 2 6839 6994 7039 6669 6885 145.23 2.1% Sample 2 6839 6994 7039 6669 6885 145.23 2.1% Sample 3 24157 24884 22207 21600 23212 1350.66 5.8% Sample 3 24157 24884 22207 21600 23212 1350.66 5.8%

Gene Name

ACE

Gene ID

1636

UniProt

P12821

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L30-L1261

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin) and saliva.

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1278 6839 24157 1358 6350 25528 Standard deviation 62.62 465.05 1087.06 86.57 482.6 1225.34 CV (%) 4.9% 6.8% 4.5% 6.5% 7.6% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1278 6839 24157 1358 6350 25528 Standard deviation 62.62 465.05 1087.06 86.57 482.6 1225.34 CV (%) 4.9% 6.8% 4.5% 6.5% 7.6% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1278 6839 24157 1358 6350 25528 Mean (pg/ml) 1278 6839 24157 1358 6350 25528 Standard deviation 62.62 465.05 1087.06 86.57 482.6 1225.34 Standard deviation 62.62 465.05 1087.06 86.57 482.6 1225.34 CV (%) 4.9% 6.8% 4.5% 6.5% 7.6% 4.8% CV (%) 4.9% 6.8% 4.5% 6.5% 7.6% 4.8%

Sensitivity

<5 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

53750 MW

Specificity

Natural and recombinant human ACE

Anticoagulant

Heparin

Gene Name Synonym

Angiotensin I converting enzyme

Protein Name

Angiotensin-converting enzyme

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.037 0.109 0.201 0.357 0.614 1.113 1.750 2.023 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.037 0.109 0.201 0.357 0.614 1.113 1.750 2.023 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.037 0.109 0.201 0.357 0.614 1.113 1.750 2.023 O.D. 0.037 0.109 0.201 0.357 0.614 1.113 1.750 2.023

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.