- Catalog number
- FEK0827
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as “AGER”. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB) .
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 153 141 152 150 5.24 3.4% Sample 2 623 570 627 596 604 22.96 3.8% Sample 3 3076 3035 3093 3144 3087 39.08 1.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 153 141 152 150 5.24 3.4% Sample 1 154 153 141 152 150 5.24 3.4% Sample 2 623 570 627 596 604 22.96 3.8% Sample 2 623 570 627 596 604 22.96 3.8% Sample 3 3076 3035 3093 3144 3087 39.08 1.2% Sample 3 3076 3035 3093 3144 3087 39.08 1.2%
- Synonyms
- CDNA FLJ56412, highly similar to Advanced glycosylation end product-specific receptor
- Gene Name
- AGER
- Gene ID
- 177
- UniProt
- B4DNX3
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q24-A344
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 78 pg/mL - 5,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 623 3076 159 595 3344 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 623 3076 159 595 3344 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 154 623 3076 159 595 3344 Mean (pg/ml) 154 623 3076 159 595 3344 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5% CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 45980 MW
- Specificity
- Natural and recombinant human Rage
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Advanced glycosylation end product-specific receptor
- Protein Name
- Advanced glycosylation end product-specific receptor
- Sample Data
- Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.037 0.116 0.191 0.330 0.611 1.112 1.761 2.023 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.037 0.116 0.191 0.330 0.611 1.112 1.761 2.023 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.037 0.116 0.191 0.330 0.611 1.112 1.761 2.023 O.D. 0.037 0.116 0.191 0.330 0.611 1.112 1.761 2.023
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
