Human CD32 PicoKine® Quick ELISA Kit

About this product

Human Fc gamma RIIB/C (CD32b/c) (FCGR2B/C) PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human FCGR2B/C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1312

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 125 105 106 114 9.06 7.9% Sample 2 404 361 357 357 369 19.84 5.3% Sample 3 1372 1213 1353 1369 1326 66.06 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 125 105 106 114 9.06 7.9% Sample 1 122 125 105 106 114 9.06 7.9% Sample 2 404 361 357 357 369 19.84 5.3% Sample 2 404 361 357 357 369 19.84 5.3% Sample 3 1372 1213 1353 1369 1326 66.06 4.9% Sample 3 1372 1213 1353 1369 1326 66.06 4.9%

Synonyms

Low affinity immunoglobulin gamma Fc region receptor II-c; IgG Fc receptor II-c; CDw32; Fc-gamma RII-c; Fc-gamma-RIIc; FcRII-c; CD32; FCGR2C; CD32, FCG2, IGFR2; FCGR2B

Gene Name

FCGR2B

Gene ID

2213/9103

UniProt

P31994/P31995

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A46-P217

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

46.9 pg/mL - 3,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 404 1372 133 365 1478 Standard deviation 8.05 19.79 97.41 9.7 22.26 130.06 CV (%) 6.6% 4.9% 7.1% 7.3% 6.1% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 404 1372 133 365 1478 Standard deviation 8.05 19.79 97.41 9.7 22.26 130.06 CV (%) 6.6% 4.9% 7.1% 7.3% 6.1% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 122 404 1372 133 365 1478 Mean (pg/ml) 122 404 1372 133 365 1478 Standard deviation 8.05 19.79 97.41 9.7 22.26 130.06 Standard deviation 8.05 19.79 97.41 9.7 22.26 130.06 CV (%) 6.6% 4.9% 7.1% 7.3% 6.1% 8.8% CV (%) 6.6% 4.9% 7.1% 7.3% 6.1% 8.8%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

35578 MW

Specificity

Natural and recombinant human FCGR2B/C

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Fc fragment of IgG receptor IIb/c

Protein Name

Low affinity immunoglobulin gamma Fc region receptor II-b/c

Sample Data

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.054 0.125 0.199 0.337 0.619 1.143 1.687 2.050 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.054 0.125 0.199 0.337 0.619 1.143 1.687 2.050 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.054 0.125 0.199 0.337 0.619 1.143 1.687 2.050 O.D. 0.054 0.125 0.199 0.337 0.619 1.143 1.687 2.050

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.