- Catalog number
- FEK1432
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 91 93 93 92 92 0.82 0.8% Sample 2 361 310 365 327 340 23.09 6.7% Sample 3 1121 1338 1162 1204 1206 81.53 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 91 93 93 92 92 0.82 0.8% Sample 1 91 93 93 92 92 0.82 0.8% Sample 2 361 310 365 327 340 23.09 6.7% Sample 2 361 310 365 327 340 23.09 6.7% Sample 3 1121 1338 1162 1204 1206 81.53 6.7% Sample 3 1121 1338 1162 1204 1206 81.53 6.7%
- Synonyms
- Dickkopf homolog 1 (Xenopus laevis) (Predicted) ; Dickkopf-like protein 1 ; Protein Dkk1 ; Dkk1 ; DKK1 , Dkk1_predicted , LOC100912050 ; rCG_48380
- Gene Name
- Dkk1
- Gene ID
- 293897
- UniProt
- D3Z9J1
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S23-H270
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 46.9 pg/mL - 3,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 91 361 1121 84 359 1024 Standard deviation 6.46 28.51 88.4%9 7.39 38.4%8 86.01 CV (%) 7.1% 7.9% 7.6% 8.8% 9.8% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 91 361 1121 84 359 1024 Standard deviation 6.46 28.51 88.4%9 7.39 38.4%8 86.01 CV (%) 7.1% 7.9% 7.6% 8.8% 9.8% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 91 361 1121 84 359 1024 Mean (pg/ml) 91 361 1121 84 359 1024 Standard deviation 6.46 28.51 88.4%9 7.39 38.4%8 86.01 Standard deviation 6.46 28.51 88.4%9 7.39 38.4%8 86.01 CV (%) 7.1% 7.9% 7.6% 8.8% 9.8% 8.4% CV (%) 7.1% 7.9% 7.6% 8.8% 9.8% 8.4%
- Sensitivity
- <15 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Calculated Molecular Weight
- 29149 MW
- Specificity
- Natural and recombinant rat DKK1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Dickkopf WNT signaling pathway inhibitor 1
- Protein Name
- Dickkopf homolog 1
- Sample Data
- Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.010 0.089 0.136 0.243 0.464 0.862 1.376 2.000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.010 0.089 0.136 0.243 0.464 0.862 1.376 2.000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.010 0.089 0.136 0.243 0.464 0.862 1.376 2.000 O.D. 0.010 0.089 0.136 0.243 0.464 0.862 1.376 2.000
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
