- Catalog number
- FEK0808
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CSF1R (Colony stimulating factor 1 receptor), also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on long arm of chromosome 5 (5q32) on the Crick (minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 106 110 121 107 111 5.95 5.3% Sample 2 398 456 393 395 410 26.32 6.4% Sample 3 1799 1871 1812 1936 1854 54.31 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 106 110 121 107 111 5.95 5.3% Sample 1 106 110 121 107 111 5.95 5.3% Sample 2 398 456 393 395 410 26.32 6.4% Sample 2 398 456 393 395 410 26.32 6.4% Sample 3 1799 1871 1812 1936 1854 54.31 2.9% Sample 3 1799 1871 1812 1936 1854 54.31 2.9%
- Synonyms
- Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; CSF-1R; M-CSF-R;2.7.10.1; Proto-oncogene c-Fms; CD115; Csf1r; Csfmr, Fms
- Gene Name
- CSF1R
- Gene ID
- 12978
- UniProt
- P09581
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A20-S511
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 106 398 1799 110 427 1748 Standard deviation 8.16 24.27 131.32 10.23 32.87 137.74 CV (%) 7.7% 6.1% 7.3% 9.3% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 106 398 1799 110 427 1748 Standard deviation 8.16 24.27 131.32 10.23 32.87 137.74 CV (%) 7.7% 6.1% 7.3% 9.3% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 106 398 1799 110 427 1748 Mean (pg/ml) 106 398 1799 110 427 1748 Standard deviation 8.16 24.27 131.32 10.23 32.87 137.74 Standard deviation 8.16 24.27 131.32 10.23 32.87 137.74 CV (%) 7.7% 6.1% 7.3% 9.3% 7.7% 7.8% CV (%) 7.7% 6.1% 7.3% 9.3% 7.7% 7.8%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Calculated Molecular Weight
- 109179 MW
- Specificity
- Natural and recombinant mouse CSF1R
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Macrophage colony-stimulating factor 1 receptor
- Protein Name
- Macrophage colony-stimulating factor 1 receptor
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.116 0.162 0.279 0.494 0.835 1.317 1.992 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.116 0.162 0.279 0.494 0.835 1.317 1.992 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.116 0.162 0.279 0.494 0.835 1.317 1.992 O.D. 0.041 0.116 0.162 0.279 0.494 0.835 1.317 1.992
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
