- Catalog number
- FEK0740
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 34 32 29 31 1.8 5.8% Sample 2 182 204 199 194 194 8.16 4.2% Sample 3 491 507 522 441 490 30.47 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 34 32 29 31 1.8 5.8% Sample 1 31 34 32 29 31 1.8 5.8% Sample 2 182 204 199 194 194 8.16 4.2% Sample 2 182 204 199 194 194 8.16 4.2% Sample 3 491 507 522 441 490 30.47 6.2% Sample 3 491 507 522 441 490 30.47 6.2%
- Synonyms
- C-X-C motif chemokine 13; B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13; Cxcl13; Blc, Scyb13
- Gene Name
- CXCL13
- Gene ID
- 55985
- UniProt
- O55038
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: I22-A109
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Calculated Molecular Weight
- 11927 MW
- Specificity
- Natural and recombinant mouse CXCL13
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-X-C motif chemokine 13
- Protein Name
- C-X-C motif chemokine 13
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.029 0.127 0.209 0.392 0.731 1.471 1.841 2.033 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.029 0.127 0.209 0.392 0.731 1.471 1.841 2.033 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.029 0.127 0.209 0.392 0.731 1.471 1.841 2.033 O.D. 0.029 0.127 0.209 0.392 0.731 1.471 1.841 2.033
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
